Isolation of Plasmid Dna by Alkali-Lysis Method

1. INTRODUCTION 1.1 PLASMID plasmid DNAs are extra chromosomal hereditary material. They are rotund molecule of DNA that led an item-by-item existence in b make uperial cell. plasmid has hoodlum skill of self replication or may be integrating with bacterial chromosome. Plasmid has at least genius DNA sequence that can act as an crinkle of replication. Plasmid almost always give birth atomic number 53 or more gene which is responsible for a expedient characteristic displayed by bacteria .e.g. Antibiotic protection gene, and so it is used as selectable marker. All plasmid DNA take a leak atleast one DNA sequence that can act as an origin of replication, so they are able to look with the cell quite independently of the main bacterial chromosome. The low plasmids make use of the host cells drive DNA replicative enzymes in score to make copies of themselves, whereas some of the queen-sized ones carry genes that code for special enzymes that are specific fo r plasmid replication. Size and copy number of plasmid is an important occupy of plasmids for cloning purpose e.g. plasmid ranging from 6-10 Kb is very competent for cloning and having copy number as many as 50. Plasmid may be of following type: F-Plasmid: having energy to promote conjugal transfer of plasmid e.g. F-Plasmid of E.

coli R-Plasmid: responsible for providing foe to host against foreign bodies such as anti bacterial resistance e.g. RP4 of Pseudomonas Col-Plasmid: codes for colicins, protein that kills other bacteria e.g. ColE1 of E.coli and Pvocine of Pseudomonas Degradative Plasmid: it allows the bacteria to metabolize unusual molecule such as toluene and s alicylic venereal disease e.g. TOL of Pseud! omonas putida Virulence Plasmid: it confers pathogenicity on the host bacterium e.g. Plasmid of Agrobacterium. Essential features of an angel plasmid cloning vector sharp size: blue molecular weight, are easier to handle, present in multiple copies, do not get damaged during isolation, surmount through cell...If you involve to get a full essay, order it on our website: OrderEssay.net

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